Rapid Detection of Avian Infectious Bronchitis Virus by Using Commercial Insulated Isothermal Polymerase Chain Reaction (iiPCR) and Enzyme-Linked Immunosorbent Assay (ELISA)

Infectious bronchitis (IB) is considered one of the most important poultry diseases worldwide. Many cases have been presented in Sri Lanka with similar clinical symptoms to those IB belt areas. Therefore, present study aimed develop IBV rapid detection methods using commercial insulated isothermal Polymerase Chain Reaction (iiPCR) and enzyme-linked immunosorbent assay (ELISA) broilers, layers, backyard flocks Lanka. Tracheal swabs blood samples (n=413) were collected from vaccinated (n=107) unvaccinated (n=306) birds exhibiting signs attributable IBV. Viral RNA was extracted tracheal detected by iiPCR. Serum separated samples, ELISA antibodies against It reported that 46% (190/413) serologically positive for 41.88% (173/413) The total prevalence 20.56% (22/107) 49.34% (151/306) Furthermore, 30.84% (33/107) 51.30% (157/306) flocks. Thus, more prevalent comprised chickens broilers sampled Moreover, Batticaloa district had highest (>75%). iiPCR could be used infection requirements.